Problem solving subject to constraints is the hallmark of engineering design (1). We adopt a design approach to invent new methods for interrogating cells and biomolecules. Our goal is to develop bioassays that are sensitive, quantitative, and as high-throughput and multiplex as possible. Most importantly, they should be reliable, generalizable, and shareable.

In the realm of signal transduction, we have developed and expanded high-throughput methods for profiling protein phosphatase activities (2, 3). These methods are now being applied to chronic viral infections along with a technique under development for a multiplex kinase activity assay. We also seek to extend or standardize established methods. For example, we have scaled quantitative real-time PCR to the entire human signaling receptome (4) and set standards for quantitative immunoblotting (5). Beyond empirical methods, we have completed a software platform for digital segmentation of brightfield images of 3D organoid cultures (to be submitted).

Selected Publications

1. 838 [Article]
2. 839 [Article]
3. 846 [Article]
4. 853 [Article]
5. 851 [Article]