Computational Protocols
Computational Protocols
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Cell Biology
Immunofluorescence on cells in suspension
[PDF]
Immunofluorescence on coverslips [PDF]
Immunofluorescence on FFPE sections [PDF]
RNA fluorescence in situ hybridization on cryosections [PDF]
Flow cytometry [PDF]
Differentiation of AC16 cells [PDF]
Preparation of amphotropic retroviruses and lentiviruses [PDF]
Viral transduction and selection of mammalian cells [PDF]
Preparation and titering of coxsackievirus B3 [PDF]
Immunofluorescence on coverslips [PDF]
Immunofluorescence on FFPE sections [PDF]
RNA fluorescence in situ hybridization on cryosections [PDF]
Flow cytometry [PDF]
Differentiation of AC16 cells [PDF]
Preparation of amphotropic retroviruses and lentiviruses [PDF]
Viral transduction and selection of mammalian cells [PDF]
Preparation and titering of coxsackievirus B3 [PDF]
Lab-specific protocols
Molecular Biology
Microplate microBCA protein assay
[PDF]
Proximity Ligation [PDF]
Western blotting for chemiluminescence [PDF]
Real-time quantitative PCR [PDF]
In vitro transcription of haptenylated riboprobes for RNA FISH [PDF]
Gateway LR recombination [PDF]
Microplate BCA protein assay [PDF]
PCR cloning [PDF]
Preparation of electrocompetent bacteria [PDF]
Production and purification of GST fusion proteins [PDF]
Receptome profiling [PDF]
shRNA cloning into pLKO.1 vectors [PDF]
Western blotting on the Licor Odyssey [PDF]
Transformation of chemically competent bacteria [PDF]
RNA purification and first-strand cDNA synthesis [PDF]
Proximity Ligation [PDF]
Western blotting for chemiluminescence [PDF]
Real-time quantitative PCR [PDF]
In vitro transcription of haptenylated riboprobes for RNA FISH [PDF]
Gateway LR recombination [PDF]
Microplate BCA protein assay [PDF]
PCR cloning [PDF]
Preparation of electrocompetent bacteria [PDF]
Production and purification of GST fusion proteins [PDF]
Receptome profiling [PDF]
shRNA cloning into pLKO.1 vectors [PDF]
Western blotting on the Licor Odyssey [PDF]
Transformation of chemically competent bacteria [PDF]
RNA purification and first-strand cDNA synthesis [PDF]
Molecular Biology Recipes
Specialized Protocols
FISH riboprobes
[PDF]
Matrigel Handling [PDF]
Small-sample cDNA amplification from microdissected cells [PDF]
Embedding of fresh brain samples for LCM [PDF]
Embedding of fresh frozen 3D structures for LCM [PDF]
Fluorescence-guided laser-capture microdissection [PDF]
Laser-capture microdissection of 3D structures [PDF]
Sectioning of fresh brain samples for LCM [PDF]
Sectioning of fresh frozen 3D structures for LCM [PDF]
Small-sample cDNA amplification [PDF]
Small-sample cDNA reamplification and labeling [PDF]
Small-sample cDNA reamplification for RNA-seq [PDF]
Subcellular phosphatase activity assay [PDF]
Matrigel Handling [PDF]
Small-sample cDNA amplification from microdissected cells [PDF]
Embedding of fresh brain samples for LCM [PDF]
Embedding of fresh frozen 3D structures for LCM [PDF]
Fluorescence-guided laser-capture microdissection [PDF]
Laser-capture microdissection of 3D structures [PDF]
Sectioning of fresh brain samples for LCM [PDF]
Sectioning of fresh frozen 3D structures for LCM [PDF]
Small-sample cDNA amplification [PDF]
Small-sample cDNA reamplification and labeling [PDF]
Small-sample cDNA reamplification for RNA-seq [PDF]
Subcellular phosphatase activity assay [PDF]
Three-dimensional breast epithelial culture
Matrigel handling
[PDF]
Whole-mount immunofluoresence of MCF10A 3D structures [PDF]
Chromatin immunoprecipitation under 3D-mimetic conditions [PDF]
Embedding of fixed frozen 3D structures [PDF]
Sectioning of fixed frozen of 3D structures [PDF]
Live-cell imaging 3D cultures by laser-scanning confocal microscopy [PDF]
Imaging 3D cultures by brightfield microscopy [PDF]
Whole-mount immunofluoresence of MCF10A 3D structures [PDF]
Chromatin immunoprecipitation under 3D-mimetic conditions [PDF]
Embedding of fixed frozen 3D structures [PDF]
Sectioning of fixed frozen of 3D structures [PDF]
Live-cell imaging 3D cultures by laser-scanning confocal microscopy [PDF]
Imaging 3D cultures by brightfield microscopy [PDF]
Video tutorials
Stochastic Profiling Hands-on Workshop
-
- Introductory Lecture [PDF] [Video]
- Sample Preparation [PDF] [Video]
- Laser-capture Microdissection and Poly(A) Amplification [PDF] [Video]
- Quantitative PCR with Stochastic Profiling Samples [PDF] [Video]
- Troubleshooting [PDF]
- Reamplification and Purification for RNA-seq [PDF] [Video]
- Monte Carlo Simulation [PDF] [Video]
- Array Processing [PDF] [Video]
- Maximum Likelihood Inference [PDF] [Video]
- Applications [PDF Part 1] [PDF Part 2] [Video]
- Hands-on Research Update [Video]
